human cd66b–fitc antibody Search Results


fitc-conjugated mouse monoclonal antibody (ab) against human cd66b  (Izasa S.A)
90
Izasa S.A fitc-conjugated mouse monoclonal antibody (ab) against human cd66b
( A ) Neutrophils (2×10 6 ) from allergic patients (n = 10) were left untreated (No adds), treated with LPS (1 µg/ml) or an Ag (G 3 ) to which the patient was sensitized (40 µg/ml) for 24 h. ( B ) and ( C ) Neutrophils from allergic patients (n = 10) were incubated with an Ag (G 3 ) to which the patient was sensitized for the indicated doses (for 24 h) or times (at a dose of 40 µg/ml), and <t>CD66b</t> cell-surface expression was analyzed by flow cytometry. The results shown are the means ± SD.
Fitc Conjugated Mouse Monoclonal Antibody (Ab) Against Human Cd66b, supplied by Izasa S.A, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd66b%E2%80%93fitc+antibody/fitc+conjugated+mouse+monoclonal+antibody++ab++against+human+cd66b/pmc03989194-33-8-17
Average 90 stars, based on 1 article reviews
fitc-conjugated mouse monoclonal antibody (ab) against human cd66b - by Bioz Stars, 2026-10
90/100 stars
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fitc anti-human cd66b antibody  (Elabscience Biotechnology)
N/A
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to
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anti-human cd66b ceacam8 monoclonal antibody fitc conjugated, flow validated  (Boster Bio)
N/A
Boster Bio Anti-Human CD66b CEACAM8 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC05978-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
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anti human cd66b antibody clone g10f5 fitc  (STEMCELL Technologies)
N/A
Isotype Note IgM kappa Host Species Note Mouse Reactivity Note Human Chimpanzee
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( A ) Neutrophils (2×10 6 ) from allergic patients (n = 10) were left untreated (No adds), treated with LPS (1 µg/ml) or an Ag (G 3 ) to which the patient was sensitized (40 µg/ml) for 24 h. ( B ) and ( C ) Neutrophils from allergic patients (n = 10) were incubated with an Ag (G 3 ) to which the patient was sensitized for the indicated doses (for 24 h) or times (at a dose of 40 µg/ml), and CD66b cell-surface expression was analyzed by flow cytometry. The results shown are the means ± SD.

Journal: PLoS ONE

Article Title: Immunotherapy Reduces Allergen-Mediated CD66b Expression and Myeloperoxidase Levels on Human Neutrophils from Allergic Patients

doi: 10.1371/journal.pone.0094558

Figure Lengend Snippet: ( A ) Neutrophils (2×10 6 ) from allergic patients (n = 10) were left untreated (No adds), treated with LPS (1 µg/ml) or an Ag (G 3 ) to which the patient was sensitized (40 µg/ml) for 24 h. ( B ) and ( C ) Neutrophils from allergic patients (n = 10) were incubated with an Ag (G 3 ) to which the patient was sensitized for the indicated doses (for 24 h) or times (at a dose of 40 µg/ml), and CD66b cell-surface expression was analyzed by flow cytometry. The results shown are the means ± SD.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody (Ab) against human CD66b, and FITC-conjugated IgG isotype control were from Coulter-Izasa (Barcelona, Spain).

Techniques: Incubation, Expressing, Flow Cytometry

( A ) Neutrophils (2×10 6 ) from allergic patients (n = 10) sensitized to G 3 but not to D 1 and W 6 , were incubated with 40 µg/ml protein extracts of G 3 , D 1 , and W 6 for 24 h. ( B ) Neutrophils from healthy subject (n = 10) were incubated with 40 µg/ml of T 9 , and G 3 for 24 h. CD66b cell-surface expression was measured by flow cytometry. The values shown are the means ± SD.

Journal: PLoS ONE

Article Title: Immunotherapy Reduces Allergen-Mediated CD66b Expression and Myeloperoxidase Levels on Human Neutrophils from Allergic Patients

doi: 10.1371/journal.pone.0094558

Figure Lengend Snippet: ( A ) Neutrophils (2×10 6 ) from allergic patients (n = 10) sensitized to G 3 but not to D 1 and W 6 , were incubated with 40 µg/ml protein extracts of G 3 , D 1 , and W 6 for 24 h. ( B ) Neutrophils from healthy subject (n = 10) were incubated with 40 µg/ml of T 9 , and G 3 for 24 h. CD66b cell-surface expression was measured by flow cytometry. The values shown are the means ± SD.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody (Ab) against human CD66b, and FITC-conjugated IgG isotype control were from Coulter-Izasa (Barcelona, Spain).

Techniques: Incubation, Expressing, Flow Cytometry

( A ) Neutrophils (2×10 6 ) from allergic patients sensitized to G 3 (n = 10) were treated, where indicated, to elute the Igs from the cell surface, and then incubated with G 3 (40 µg/ml) or LPS (1 µg/ml) for 24 h, and CD66 cell-surface expression was measured by flow cytometry. Specific IgE and IgG for the above Ags were determined in the elution solution by ELISA. ( B ) Neutrophils from allergic patients (n = 5) were incubated with anti-FcεRI (CRA 1), anti-CD23 (9P.25) or anti-galectin-3 (A3A12) antibodies at 5 µg/ml for 24 h, and CD66b cell-surface expression was measured as in A. The values shown are the means ± SD.

Journal: PLoS ONE

Article Title: Immunotherapy Reduces Allergen-Mediated CD66b Expression and Myeloperoxidase Levels on Human Neutrophils from Allergic Patients

doi: 10.1371/journal.pone.0094558

Figure Lengend Snippet: ( A ) Neutrophils (2×10 6 ) from allergic patients sensitized to G 3 (n = 10) were treated, where indicated, to elute the Igs from the cell surface, and then incubated with G 3 (40 µg/ml) or LPS (1 µg/ml) for 24 h, and CD66 cell-surface expression was measured by flow cytometry. Specific IgE and IgG for the above Ags were determined in the elution solution by ELISA. ( B ) Neutrophils from allergic patients (n = 5) were incubated with anti-FcεRI (CRA 1), anti-CD23 (9P.25) or anti-galectin-3 (A3A12) antibodies at 5 µg/ml for 24 h, and CD66b cell-surface expression was measured as in A. The values shown are the means ± SD.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody (Ab) against human CD66b, and FITC-conjugated IgG isotype control were from Coulter-Izasa (Barcelona, Spain).

Techniques: Incubation, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

CD66b expression is higher on neutrophils from NLP than those from peripheral blood. ( A ) Neutrophils (2×10 6 cells) from healthy subjects (n = 10), non-IT-treated patients with allergic asthma and rhinitis sensitized to G 3 (n = 10), and IT-treated patients with allergic asthma and rhinitis sensitized to G 3 (n = 15) were left untreated (No adds) or treated with G 3 (40 µg/ml) for 24 h. ( B ) Neutrophils purified from peripheral blood and NLF from non-IT treated patients. The levels of CD66b cell-surface expression were measured by flow cytometry. The values shown are the means ± SD.

Journal: PLoS ONE

Article Title: Immunotherapy Reduces Allergen-Mediated CD66b Expression and Myeloperoxidase Levels on Human Neutrophils from Allergic Patients

doi: 10.1371/journal.pone.0094558

Figure Lengend Snippet: CD66b expression is higher on neutrophils from NLP than those from peripheral blood. ( A ) Neutrophils (2×10 6 cells) from healthy subjects (n = 10), non-IT-treated patients with allergic asthma and rhinitis sensitized to G 3 (n = 10), and IT-treated patients with allergic asthma and rhinitis sensitized to G 3 (n = 15) were left untreated (No adds) or treated with G 3 (40 µg/ml) for 24 h. ( B ) Neutrophils purified from peripheral blood and NLF from non-IT treated patients. The levels of CD66b cell-surface expression were measured by flow cytometry. The values shown are the means ± SD.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody (Ab) against human CD66b, and FITC-conjugated IgG isotype control were from Coulter-Izasa (Barcelona, Spain).

Techniques: Expressing, Purification, Flow Cytometry

( A ) CD66b expression was analyzed on cells from NLF in healthy subjects (n = 10), IT-treated allergic patients (n = 15) and non-IT-treated allergic patients (n = 10). ( B ) MPO activity was analyzed in NLF from healthy subjects (n = 10), IT-treated allergic patients (n = 15) and non-IT-treated allergic patients (n = 10). The values shown are the mean ± SD.

Journal: PLoS ONE

Article Title: Immunotherapy Reduces Allergen-Mediated CD66b Expression and Myeloperoxidase Levels on Human Neutrophils from Allergic Patients

doi: 10.1371/journal.pone.0094558

Figure Lengend Snippet: ( A ) CD66b expression was analyzed on cells from NLF in healthy subjects (n = 10), IT-treated allergic patients (n = 15) and non-IT-treated allergic patients (n = 10). ( B ) MPO activity was analyzed in NLF from healthy subjects (n = 10), IT-treated allergic patients (n = 15) and non-IT-treated allergic patients (n = 10). The values shown are the mean ± SD.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody (Ab) against human CD66b, and FITC-conjugated IgG isotype control were from Coulter-Izasa (Barcelona, Spain).

Techniques: Expressing, Activity Assay

( A ) The percentage of CD66b + MPO + cells was analyzed on cells from NLF in healthy subjects (n = 10), IT-treated allergic patients (n = 15) and non-IT-treated allergic patients (n = 10). The values shown are the mean ± SD. ( B ) Three representative dot-blot of the results shown in A.

Journal: PLoS ONE

Article Title: Immunotherapy Reduces Allergen-Mediated CD66b Expression and Myeloperoxidase Levels on Human Neutrophils from Allergic Patients

doi: 10.1371/journal.pone.0094558

Figure Lengend Snippet: ( A ) The percentage of CD66b + MPO + cells was analyzed on cells from NLF in healthy subjects (n = 10), IT-treated allergic patients (n = 15) and non-IT-treated allergic patients (n = 10). The values shown are the mean ± SD. ( B ) Three representative dot-blot of the results shown in A.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody (Ab) against human CD66b, and FITC-conjugated IgG isotype control were from Coulter-Izasa (Barcelona, Spain).

Techniques: Dot Blot